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Henan Tongwei Medical Equipment Co., LtdCall us : +86 − −19139704654 // Email us : [email protected]
Henan Tongwei Medical Equipment Co., LtdCall us : +86 − −19139704654 // Email us : [email protected]
Apr 03 2007library inserts are expressed as GAL4 fusions with c-Myc and hemagglutinin (HA) epitope tags respectively The epitope tags eliminate the need to generate specificantibodies to each new protein and allow convenient identification of the fusion proteins System 3 vectors also include T7 promoters downstream of the GAL4 coding sequences These
Aug 25 2010The coding sequence for HA epitope (represented in red) was added by PCR at the 3' end of full-length cDNA B) Cells transfected with either mouse Rai1-HA plasmid (Rai1-HA) the human RAI1 plasmid RAI1 or RAI1-HA an empty vector (e/v) or untransfected cells (u/t) were lysed and a western blot analysis was performed with an anti HA antibody
PSF-CMV-PURO-NH2-6HIS-HA-THR - N-TERMINAL 6 HIS AND HA DUAL TAG MAMMALIAN PLASMID plasmid vector for molecular cloning Synonym: cloning vector expression vector molecular cloning vector plasmid plasmid vector snapfast vector vector find Sigma-Aldrich-OGS1203 MSDS related peer-reviewed papers technical documents similar products more at Sigma-Aldrich
The inducible expression cassette contains a multiple cloning site that contains three contiguous copies of the HA epitope(3 HA) positioned for fusion at the C-terminus of the protein of interest A second expression cassette in which the hygromycin-resistance gene is expressed from the TK promoter is located downstream (relative to
About the Peptide Tag:PSF-TEF1-NH2-HA-FXA - N-terminal HA TAG yeast plasmid is an N-terminal Influenza Hemagglutinin (HA) epitope tag yeast expression plasmid for molecular cloning that can be fused to a gene of interest to allow protein detection and/or purification This plasmid vector allows the creation of tagged proteins with a FXa
HA epitope-tagged CCR2a (1 6 mg/well) or human Flag epitope-tagged CCR2b (1 6 mg/well) Transfected cells were pelleted by centrifugation and resuspended in 150 ml/well ice-cold lysis buffer containing 20 mM Tris/HCl pH 7 5 1 mM EDTA 3 mM GDP 2 g/ml soybean trypsin inhibitor 1 M pepstatin 1 mM leupeptin 100 mM PMSF and 1 mg/ml aprotinin
Live attenuated strains of Salmonella enterica have a high potential as carriers of recombinant vaccines The type III secretion system (T3SS)-dependent translocation of S enterica can be deployed for delivery of heterologous antigens to antigen-presenting cells Here we investigated the efficacy of various effector proteins of the Salmonella pathogenicity island (SPI2)-encoded T3SS for the
Additionally a C terminal HA epitope tag aids detection isolation and purification of the Cas9 protein This mRNA is capped using CleanCap our proprietary co-transciptional capping method which results in the naturally occuring Cap 1 structure with high capping efficiency
Live attenuated strains of Salmonella enterica have a high potential as carriers of recombinant vaccines The type III secretion system (T3SS)-dependent translocation of S enterica can be deployed for delivery of heterologous antigens to antigen-presenting cells Here we investigated the efficacy of various effector proteins of the Salmonella pathogenicity island (SPI2)-encoded T3SS for the
Epitope tags are peptide or protein sequences covalently conjugated usually to the C- or N-terminus of a recombinant protein The addition of epitope tags allows researchers to better identify and purify a target protein within a cell lysate or localize it and assess its expression in intact cells when studying transfectants expressing the fusion protein
Mac1-(1–194) was excised form Mac1-(1–194)/VP16 as a AflIII/NotI fragment and subcloned into a derivative of pET21d which contained sequences coding for a single copy of the HA epitope (pET21HA) generating a C-terminal fusion of the HA epitope with Mac1-(1–194) Mutations in the two Cys pairs in the N-terminal 194-residue C69S C70S and
Apr 25 2012The intronic GABRG2 mutation IVS6+2T→G was identified in an Australian family with childhood absence epilepsy and febrile seizures ([Kananura et al 2002][1]) The GABRG2 intron 6 splice donor site was found to be mutated from GT to GG We generated wild-type and mutant γ2 subunit bacterial artificial chromosomes (BACs) driven by a CMV promoter and expressed them in HEK293T
Epitope Tag and Reporter Protein Antibodies The use of epitope tags in recombinant DNA techniques allows the detection of proteins where specific antibodies are not available This eliminates the need to generate a new antibody for each protein saving time resources and money
Unique among epitope tags GFP is an autofluorescent 27 kDa tag that can be directly detected in living cells by fluorescent microscopy Hemagglutinin A (HA) Derived from the binding domain of the Influenza hemagglutinin protein HA contains a high proportion of charged residues ( YPYDVPDYA ) so it is likely to form a strong antibody
3 1/HA (Fig 2) A band of identical size was present in lysates of LNCaP cells transfected with NKX-3 1/HA(Fig 2 Input) confirming that this band represents the HA epitope-tagged NKX-3 1 protein Immunoprecipitation with antimouse PDEF polyclonal antibodies also brought down a complex containing NKX-3 1/HA exclusively in cotransfected cells
3 1/HA (Fig 2) A band of identical size was present in lysates of LNCaP cells transfected with NKX-3 1/HA(Fig 2 Input) confirming that this band represents the HA epitope-tagged NKX-3 1 protein Immunoprecipitation with antimouse PDEF polyclonal antibodies also brought down a complex containing NKX-3 1/HA exclusively in cotransfected cells
Previous studies have shown that the synthetic peptide GK1 derived from Taenia crassiceps cysticerci enhances the immunogenicity of the commercial inactivated influenza vaccine Fluzone in both young and aged mice In particular antibody responses were much improved Since GK1 is a peptide and is rapidly cleared from the body it offers the possibility to improve vaccine performance without
Unique among epitope tags GFP is an autofluorescent 27 kDa tag that can be directly detected in living cells by fluorescent microscopy Hemagglutinin A (HA) Derived from the binding domain of the Influenza hemagglutinin protein HA contains a high proportion of charged residues ( YPYDVPDYA ) so it is likely to form a strong antibody
This is a polyclonal rabbit anti-HA (Hemagglutinin) Tag antibody labeled with our superior CF dyes The antibody recognizes the hemagglutinin (HA) epitope tag (YPYDVPDYA) Available in 4 bright and photostable CF dyes Suitable for western immunofluorescence and immunohistology in FFPE tissues
Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing Hidehiko K Inagaki 1 2 Shlomo Ben-Tabou de-Leon 1 2 Allan M Wong 1 2 Smitha Jagadish 4 Hiroshi Ishimoto 3 Gilad Barnea 5 Toshihiro Kitamoto 3 Richard Axel 1 4 and David J Anderson1 2 * 1Howard Hughes Medical Institute 2Division of Biology 156-29
This tag allows the detection and purification of a tagged protein using antibodies raised against the Influenza HA epitope The HA tag coding sequence is YPYDVPDYA There is an enterokinase cleavage site (DDDTongwei) immediately downstream of the HA tag that can be used to remove the HA tag from a purified protein It cleaves after the lysine residue
An affinity tag system requires both high affinity and specificity The RAP tag epitope DMVNPGLEDRIE derived from rat podoplanin (PDPN) is specifically recognized by PMab-2 monoclonal antibodies in rats Here we demonstrated that high levels of PMab-2 can be produced in Nicotiana benthamiana and plant-derived PMab-2 possesses similar activity to CHO-derived PMab-2 and the RAP tag presents
Apr 03 2007library inserts are expressed as GAL4 fusions with c-Myc and hemagglutinin (HA) epitope tags respectively The epitope tags eliminate the need to generate specificantibodies to each new protein and allow convenient identification of the fusion proteins System 3 vectors also include T7 promoters downstream of the GAL4 coding sequences These
Dec 17 2019Figure 1 Ability of influenza NS1 proteins to inhibit host gene expression and ISRE promoter activation: Human 293T cells (2 5 10 5 cells/well 24-well plate format triplicates) were transiently co-transfected with 25 ng of polymerase II expression pCAGGS plasmids encoding Gluc together with 1 μg of the indicated HA epitope tagged IAV (PR8 and 1918) IBV ICV and IDV NS1
Our polyclonal and monoclonal primary antibodies are directed toward some important reporter gene products (green-fluorescent protein (GFP) glutathione S-transferase (GST) glucuronidase and galactosidase) and epitope tags (oligohistidine hemagglutinin and c-myc) as well as proteins found in the cytoskeleton synaptic vesicles neurons mitochondria and yeast
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